Automated parallel DNA sequencing on multiple channel microchips
نویسندگان
چکیده
منابع مشابه
Automated parallel DNA sequencing on multiple channel microchips.
We report automated DNA sequencing in 16-channel microchips. A microchip prefilled with sieving matrix is aligned on a heating plate affixed to a movable platform. Samples are loaded into sample reservoirs by using an eight-tip pipetting device, and the chip is docked with an array of electrodes in the focal plane of a four-color scanning detection system. Under computer control, high voltage i...
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Confirmation of mutations mostly involves DNA sequencing. The conventional manual radioactive labeling sequencing protocol may be conceived as the method of choice because of the perception that the capital and operational costs of automated sequencers are high. Velickovic et al. (3) reduced the cost of DNA sequencing by running 48 or 64 samples on a 36-lane model 377-01 ABI PRISM automated se...
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T7 DNA polymerase with chemically inactivated 3'-5' exo-nuclease activity, as well as unmodified T7 DNA polymerase, were used for sequencing by the dideoxy method in an automated system with fluorescence labelled primer and on-line detection of laser-excited reaction products. An analysis of signal intensity variations in the C track revealed that low C signals were usually preceded by a T in t...
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MOTIVATION Reconstruction of the original DNA sequence in the sequencing by the hybridization approach (SBH) requires computational support due to a large number of possible combinations. One can notice a lack of algorithms admitting false-negative data and giving in addition all possible solutions. RESULTS In this paper, a new method of sequencing has been proposed. An algorithm based on its...
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Much work has been performed since the development of the lab-on-a-chip concept that has brought microfabricated systems to the forefront of bioanalytical research. The success of using these microchips for performing complicated biological assays faster and cheaper than conventional methods has facilitated their emerging popularity among researchers. A recently exploited advantage of microfabr...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2000
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.100113197